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The cytosol complex II was incubated with 3 m M of ZnCl 2 , CoCl 2 , MgCl 2 , or CaCl 2 for 20 minutes at 0°C (A) or 20°C (B) before it was layered on the top of a glycerol gradient (10 to 22%) solution and analyzed by gradient centrifugation. Centrifugation was performed at 54,000 rpm for 18 hours. The solid lines represent results with the samples containing no metal ion, or in the presence of MnCl 2 , MgCl 2 , or CaCl 2 which had no visible effect on the sedimentation patterns; (—), Zn 2+ ; (…), Co 2+. The gradient medium had pH of 7, dianabol hi tech pharmaceuticals side effects. The sedimentation was from right to left ( Liao et al. Testoviron bogactwo Activation of G proteins was determined by measuring an increase in specific binding of [ 35 S]GTPγ-S to plasma membranes as described previously ( 6, 23), dianabol hi tech pharmaceuticals side effects.
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